Esterol 14-demetilase

Esterol 14-demetilase é uma enzima que catalisa a reação química: obtusifoliol + 3 O₂ + 3 NADPH + 3 H⁺ ${\displaystyle \rightleftharpoons }$ 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formiato + 3 NADP⁺ + 4 H₂O.

Os quatro substratos desta enzima são o obtusifoliol, O₂, NADPH, e H⁺, e os quatro produtos são 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol, formiato, NADP⁺, e H₂O.

Esta enzima pertence a família das oxidoredutases e participa na biossíntese dos esteroides.

Referências

• Bak S, Kahn RA, Olsen CE, Halkier BA (1997). «Cloning and expression in Escherichia coli of the obtusifoliol 14 alpha-demethylase of Sorghum bicolor (L.) Moench, a cytochrome P450 orthologous to the sterol 14 alpha-demethylases (CYP51) from fungi and mammals». Plant. J. 11 (2): 191–201. PMID 9076987. doi:10.1046/j.1365-313X.1997.11020191.x  !CS1 manut: Nomes múltiplos: lista de autores (link)
• Aoyama Y, Yoshida Y (1991). «Different substrate specificities of lanosterol 14a-demethylase (P-45014DM) of Saccharomyces cerevisiae and rat liver for 24-methylene-24,25-dihydrolanosterol and 24,25-dihydrolanosterol». Biochem. Biophys. Res. Commun. 178 (3): 1064–71. PMID 1872829. doi:10.1016/0006-291X(91)91000-3 
• Aoyama Y, Yoshida Y (1992). «The 4 beta-methyl group of substrate does not affect the activity of lanosterol 14 alpha-demethylase (P-450(14)DM) of yeast: difference between the substrate recognition by yeast and plant sterol 14 alpha-demethylases». Biochem. Biophys. Res. Commun. 183 (3): 1266–72. PMID 1567403. doi:10.1016/S0006-291X(05)80327-4 
• DHR (1972). «The removal of the 32-carbon atom as formic acid in cholesterol biosynthesis». J. Chem. Soc. Chem. Commun.: 383–385